Section 2 - Composition, Information on Ingredients

CAS#	Chemical Name	Percent	EINECS/ELINCS
1239-45-8	Ethidium bromide	95+	214-984-6

Section 3 - Hazards Identification

Appearance: dark purple crystalline powder.
Danger! Harmful in contact with skin and if swallowed. May be fatal if inhaled. Causes eye, skin, and respiratory tract irritation. Possible risks of irreversible effects.
Target Organs: Respiratory system, eyes, skin.


Potential Health Effects
Eye: Causes eye irritation.
Skin: Causes skin irritation. Harmful if absorbed through the skin.
Ingestion: Harmful if swallowed. May cause irritation of the digestive tract.
Inhalation: May be fatal if inhaled. Causes respiratory tract irritation. Inhalation of vapors will cause coughing or breathing difficulty.
Chronic: Laboratory experiments have resulted in mutagenic effects. Possible risk of irreversible effects.

Section 4 - First Aid Measures

Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid imme diately.
Skin: Get medical aid immediately. Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes.
Ingestion: Do not induce vomiting. Get medical aid immediately. Call a poison control center.
Inhalation: Do not use mouth-to-mouth resuscitation if victim ingested or inhaled the substance; induce artificial respiration with the aid of a pocket mask equipped with a one-way valve or other proper respiratory medical device. POISON material. If inhaled, get medical aid immediately. Remove victim to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen.
Notes to Physician: Treat symptomatically and supportively.

Section 5 - Fire Fighting Measures

General Information: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear.
Extinguishing Media: Use water spray, dry chemical, carbon dioxide, or chemical foam.
Flash Point: > 100 deg C (> 212.00 deg F)
Autoignition Temperature: Not available.
Explosion Limits, Lower:Not available.
Upper: Not available.
NFPA Rating: (estimated) Health: 4; Flammability: 1; Instability: 0

Section 6 - Accidental Release Measures

General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: Vacuum or sweep up material and place into a suitable disposal container. Wear a self contained breathing apparatus and appropriate personal protection. (See Exposure Controls, Personal Protection section). Avoid generating dusty conditions. Provide ventilation. Evacuate unnecessary personnel. Do not let this chemical enter the environment.

Section 7 - Handling and Storage

Handling: Minimize dust generation and accumulation. Do not get in eyes, on skin, or on clothing. Do not ingest or inhale. Use only in a chemical fume hood.
Storage: Store in a cool, dry place. Store in a tightly closed container.

Section 8 - Exposure Controls, Personal Protection

Engineering Controls: Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use only under a chemical fume hood.
Exposure Limits

Chemical Name	ACGIH	NIOSH	OSHA - Final PELs
Ethidium bromide	none listed	none listed	none listed

OSHA Vacated PELs: Ethidium bromide: No OSHA Vacated PELs are listed for this chemical.
Personal Protective Equipment
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin: Wear appropriate protective gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: A respiratory protection program that meets OSHA's 29 CFR 1910.134 and ANSI Z88.2 requirements or European Standard EN 149 must be followed whenever workplace conditions warrant respirator use.

Section 9 - Physical and Chemical Properties

Physical State: Crystalline powder
Appearance: dark purple
Odor: low odor
pH: 4.0 - 7.0 (2% aq.sol.)
Vapor Pressure: Not available.
Vapor Density: Not available.
Evaporation Rate:Not available.
Viscosity: Not available.
Boiling Point: Not available.
Freezing/Melting Point:260 - 262 deg C(decom)
Decomposition Temperature:Not available.
Solubility: Soluble.
Specific Gravity/Density:Not available.
Molecular Formula:C21H20BrN3
Molecular Weight:394.3

Section 10 - Stability and Reactivity

Chemical Stability: Stable under normal temperatures and pressures.
Conditions to Avoid: Incompatible materials, dust generation, excess heat.
Incompatibilities with Other Materials: Strong oxidizing agents.
Hazardous Decomposition Products: Nitrogen oxides, carbon monoxide, carbon dioxide, hydrogen bromide.
Hazardous Polymerization: Will not occur.

Section 11 - Toxicological Information

RTECS#:      
CAS# 1239-45-8: SF7950000
LD50/LC50:
Not available.
LC50 (inhalation, rat): 0.0118 - 0.1340 mg/L/6H; LD50 (oral,rat): 1503 mg/kg.
Carcinogenicity:
CAS# 1239-45-8: Not listed by ACGIH, IARC, NTP, or CA Prop 65.

Epidemiology: No information available.
Teratogenicity: No information available.
Reproductive Effects: No information available.
Mutagenicity: Mutagenic effects have occurred in humans.See actual entry in RTECS for complete information.
Neurotoxicity: No information available.
Other Studies:

Section 12 - Ecological Information

Ecotoxicity: No data available. No information available.
Environmental: No information available.
Physical: No information available.
Other: Do not empty into drains.

Section 13 - Disposal Considerations

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series: None listed.

Section 14 - Transport Information

	US DOT	Canada TDG
Shipping Name:	TOXIC SOLID, ORGANIC, N.O.S.* (Ethidium bromide)	TOXIC SOLID, ORGANIC, N.O.S.* (Ethidium bromide)
Hazard Class:	6.1	6.1
UN Number:	UN2811	UN2811
Packing Group:	I	I

Section 15 - Regulatory Information

US FEDERAL

TSCA
     CAS# 1239-45-8 is not listed on the TSCA inventory. It is for research and development use only.
Health & Safety Reporting List
     None of the chemicals are on the Health & Safety Reporting List.
Chemical Test Rules
     None of the chemicals in this product are under a Chemical Test Rule.
Section 12b
     None of the chemicals are listed under TSCA Section 12b.
TSCA Significant New Use Rule
     None of the chemicals in this material have a SNUR under TSCA.
CERCLA Hazardous Substances and corresponding RQs
     None of the chemicals in this material have an RQ.
SARA Section 302 Extremely Hazardous Substances
     None of the chemicals in this product have a TPQ.
SARA Codes
     CAS # 1239-45-8: delayed.
Section 313      No chemicals are reportable under Section 313.
Clean Air Act:
     This material does not contain any hazardous air pollutants.
     This material does not contain any Class 1 Ozone depletors.
     This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
     None of the chemicals in this product are listed as Hazardous Substances under the CWA.
     None of the chemicals in this product are listed as Priority Pollutants under the CWA.
     None of the chemicals in this product are listed as Toxic Pollutants under the CWA.
OSHA:
     None of the chemicals in this product are considered highly hazardous by OSHA.
STATE
     CAS# 1239-45-8 is not present on state lists from CA, PA, MN, MA, FL, or NJ.

California Prop 65

California No Significant Risk Level: None of the chemicals in this product are listed.

European/International Regulations
European Labeling in Accordance with EC Directives
Hazard Symbols:
     T+
Risk Phrases:
     R 21/22 Harmful in contact with skin and if swallowed.
     R 26 Very toxic by inhalation.
     R 36/37/38 Irritating to eyes, respiratory system and skin.
     R 68 Possible risk of irreversible effects.

Safety Phrases:
     S 22 Do not breathe dust.
     S 26 In case of contact with eyes, rinse immediately with plenty of
     water and seek medical advice.
     S 36/37/39 Wear suitable protective clothing, gloves and eye/face pr
     otection.
     S 45 In case of accident or if you feel unwell, seek medical advice
     immediately (show the label where possible).
     S 28A After contact with skin, wash immediately with plenty of water
     .

WGK (Water Danger/Protection)
     CAS# 1239-45-8: 2
Canada - DSL/NDSL
None of the chemicals in this product are listed on the DSL or NDSL list.
Canada - WHMIS
     This product has a WHMIS classification of D1A, D2B.
This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations and the MSDS contains all of the information required by those regulations.
Canadian Ingredient Disclosure List

Section 16 - Additional Information

MSDS Creation Date: 4/08/1999
Revision #12 Date: 4/17/2008

The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Fisher be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Fisher has been advised of the possibility of such damages.