Material Safety Data Sheet
Carnoy's Solution

ACC# 45410
Section 1 - Chemical Product and Company Identification

MSDS Name: Carnoy's Solution
Catalog Numbers: S79954, S799541
Synonyms:
None.
Company Identification:

              Fisher Scientific
              1 Reagent Lane
              Fair Lawn, NJ 07410
For information, call:
201-796-7100
Emergency Number:
201-796-7100
For CHEMTREC assistance, call:
800-424-9300
For International CHEMTREC assistance, call:
703-527-3887

Section 2 - Composition, Information on Ingredients

CAS# Chemical Name Percent EINECS/ELINCS
64-17-5 Ethyl alcohol 54.42 200-578-6
67-66-3 Chloroform 30.0 200-663-8
64-19-7 Acetic acid 10.0 200-580-7
141-78-6 Ethyl acetate 3.0 205-500-4
67-56-1 Methyl alcohol 2.1 200-659-6
Aliphatic Hydrocarbons 0.48 unlisted

Section 3 - Hazards Identification

EMERGENCY OVERVIEW

Appearance: colorless liquid. Flash Point: 8.3 deg C.
Danger! Causes eye, skin, and respiratory tract irritation. Flammable liquid and vapor. This substance has caused adverse reproductive and fetal effects in humans. Aspiration hazard if swallowed. Can enter lungs and cause damage. May cause central nervous system depression. May cause cancer based on animal studies. May cause cardiac disturbances. May cause liver, kidney and heart damage. May cause blindness if swallowed.
Target Organs: Kidneys, heart, central nervous system, liver, eyes.


Potential Health Effects
Eye: Causes severe eye irritation. May cause painful sensitization to light. May cause chemical conjunctivitis and corneal damage.
Skin: Causes moderate skin irritation. May be absorbed through the skin.
Ingestion: May be fatal or cause blindness if swallowed. May cause gastrointestinal irritation with nausea, vomiting and diarrhea. May cause systemic toxicity with acidosis. May cause central nervous system depression, characterized by excitement, followed by headache, dizziness, drowsiness, and nausea. Advanced stages may cause collapse, unconsciousness, coma and possible death due to respiratory failure. May cause cardiac disturbances. Aspiration of material into the lungs may cause chemical pneumonitis, which may be fatal.
Inhalation: Inhalation of high concentrations may cause central nervous system effects characterized by nausea, headache, dizziness, unconsciousness and coma. Causes respiratory tract irritation. May cause cardiac sensitization and possible failure. May cause narcotic effects in high concentration. Vapors may cause dizziness or suffocation.
Chronic: Prolonged or repeated skin contact may cause defatting and dermatitis. Repeated exposure may cause erosion of teeth. Toxicity may be increased by exposure to alcohol, steroids, and ketones. Animal studies have reported fetal losses, delays in fetal development, and cleft palate. Laboratory experiments have resulted in mutagenic effects. Animal studies have reported the development of tumors. Prolonged exposure may cause liver, kidney, and heart damage.

Section 4 - First Aid Measures


Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid.
Skin: Get medical aid. Immediately flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Wash clothing before reuse.
Ingestion: Do not induce vomiting. If victim is conscious and alert, give 2-4 cupfuls of milk or water. Never give anything by mouth to an unconscious person. Possible aspiration hazard. Get medical aid immediately.
Inhalation: Get medical aid immediately. Remove from exposure and move to fresh air immediately. If breathing is difficult, give oxygen. Do NOT use mouth-to-mouth resuscitation. If breathing has ceased apply artificial respiration using oxygen and a suitable mechanical device such as a bag and a mask.
Notes to Physician: Causes cardiac sensitization to endogenous catelcholamines which may lead to cardiac arrhythmias. Do NOT use adrenergic agents such as epinephrine or pseudoepinephrine. Treat symptomatically and supportively. Persons with skin or eye disorders or liver, kidney, chronic respiratory diseases, or central and peripheral nervous sytem diseases may be at increased risk from exposure to this substance. Activated charcoal does not reduce ethanol absorption.
Antidote: Ethanol may inhibit methanol metabolism.

Section 5 - Fire Fighting Measures


General Information: Ethanol may inhibit methanol metabolism. As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. Vapors may form an explosive mixture with air. Vapors can travel to a source of ignition and flash back. May burn with invisible flame. Will burn if involved in a fire. Flammable Liquid. Can release vapors that form explosive mixtures at temperatures above the flashpoint. Use water spray to keep fire-exposed containers cool. Containers may explode in the heat of a fire. Vapors may be heavier than air. They can spread along the ground and collect in low or confined areas. May be ignited by heat, sparks, and flame. Vapors may form an explosive mixture with air.
Extinguishing Media: For small fires, use dry chemical, carbon dioxide, water spray or alcohol-resistant foam. For large fires, use water spray, fog, or alcohol-resistant foam. Water may be ineffective. Do NOT use straight streams of water. Cool containers with flooding quantities of water until well after fire is out.
Flash Point: 8.3 deg C ( 46.94 deg F)
Autoignition Temperature: 362.8 deg C ( 685.04 deg F)
Explosion Limits, Lower:3 vol %
Upper: 19 vol %
NFPA Rating: (estimated) Health: 1; Flammability: 3; Instability: 0

Section 6 - Accidental Release Measures


General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: Absorb spill with inert material (e.g. vermiculite, sand or earth), then place in suitable container. Clean up spills immediately, observing precautions in the Protective Equipment section. Remove all sources of ignition. Use a spark-proof tool. Provide ventilation. A vapor suppressing foam may be used to reduce vapors. Water spray may reduce vapor but may not prevent ignition in closed spaces.

Section 7 - Handling and Storage


Handling: Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Use only in a well-ventilated area. Ground and bond containers when transferring material. Use spark-proof tools and explosion proof equipment. Avoid contact with eyes, skin, and clothing. Empty containers retain product residue, (liquid and/or vapor), and can be dangerous. Keep container tightly closed. Keep away from heat, sparks and flame. Do not ingest or inhale. Do not pressurize, cut, weld, braze, solder, drill, grind, or expose empty containers to heat, sparks or open flames.
Storage: Keep away from heat, sparks, and flame. Keep away from sources of ignition. Do not store in direct sunlight. Store in a tightly closed container. Keep from contact with oxidizing materials. Store in a cool, dry, well-ventilated area away from incompatible substances. Flammables-area. Do not store near perchlorates, peroxides, chromic acid or nitric acid.

Section 8 - Exposure Controls, Personal Protection


Engineering Controls: Use explosion-proof ventilation equipment. Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate general or local exhaust ventilation to keep airborne concentrations below the permissible exposure limits.
Exposure Limits
Chemical Name ACGIH NIOSH OSHA - Final PELs
Ethyl alcohol 1000 ppm TWA 1000 ppm TWA; 1900 mg/m3 TWA 3300 ppm IDLH 1000 ppm TWA; 1900 mg/m3 TWA
Chloroform 10 ppm TWA 500 ppm IDLH 50 ppm Ceiling; 240 mg/m3 Ceiling
Acetic acid 10 ppm TWA; 15 ppm STEL 10 ppm TWA; 25 mg/m3 TWA 50 ppm IDLH 10 ppm TWA; 25 mg/m3 TWA
Ethyl acetate 400 ppm TWA 400 ppm TWA; 1400 mg/m3 TWA 2000 ppm IDLH 400 ppm TWA; 1400 mg/m3 TWA
Methyl alcohol 200 ppm TWA; 250 ppm STEL; Skin - potential significant contribution to overall exposure by the cutaneous r oute 200 ppm TWA; 260 mg/m3 TWA 6000 ppm IDLH 200 ppm TWA; 260 mg/m3 TWA
Aliphatic Hydrocarbons none listed none listed none listed

OSHA Vacated PELs: Ethyl alcohol: 1000 ppm TWA; 1900 mg/m3 TWA Chloroform: 2 ppm TWA; 9.78 mg/m3 TWA Acetic acid: 10 ppm TWA; 25 mg/m3 TWA Ethyl acetate: 400 ppm TWA; 1400 mg/m3 TWA Methyl alcohol: 200 ppm TWA; 260 mg/m3 TWA Aliphatic Hydrocarbons: No OSHA Vacated PELs are listed for this chemical.
Personal Protective Equipment
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin: Wear appropriate protective gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: A respiratory protection program that meets OSHA's 29 CFR 1910.134 and ANSI Z88.2 requirements or European Standard EN 149 must be followed whenever workplace conditions warrant respirator use.

Section 9 - Physical and Chemical Properties


Physical State: Liquid
Appearance: colorless
Odor: acetic odor
pH: Acidic.
Vapor Pressure: 100 mm Hg
Vapor Density: 4.12 (Air=1)
Evaporation Rate:11.6
Viscosity: Not available.
Boiling Point: 118 deg C
Freezing/Melting Point:-65 deg C
Decomposition Temperature:Not available.
Solubility: Soluble in water.
Specific Gravity/Density:1.0
Molecular Formula:Mixture
Molecular Weight:Not available.

Section 10 - Stability and Reactivity


Chemical Stability: Stable under normal temperatures and pressures.
Conditions to Avoid: Light, ignition sources, excess heat.
Incompatibilities with Other Materials: Metals, strong oxidizing agents, acids, alkali metals, aluminum, ammonia, fluorine, hydrazine, magnesium, peroxides, potassium, lithium, sodium, acids (mineral, non-oxidizing, e.g. hydrochloric acid, hydrofluoric acid, muriatic acid, phosphoric acid), acid anhydrides, calcium hypochlorite, dinitrogen tetraoxide, sodium + methanol, chromyl chloride, nitrosyl perchlorate, bromine pentafluoride, perchloric acid, silver nitrate, mercuric nitrate, potassium tert-butoxide, magnesium perchlorate, acid chlorides, platinum, uranium hexafluoride, silver oxide, iodine heptafluoride, acetyl bromide, disulfuryl difluoride, tetrachlorosilane + water, acetyl chloride, nitrogen tetroxide, permanganic acid, ruthenium (VIII) oxide, uranyl perchlorate, acetone + alkali, disilane, perchloric acid + phosphorus pentoxide, sodium methylate, triisopropylphosphine, sodium methoxide + methanol, potassium dioxide.
Hazardous Decomposition Products: Hydrogen chloride, carbon monoxide, irritating and toxic fumes and gases, carbon dioxide, chlorine, phosgene gas.
Hazardous Polymerization: Will not occur.

Section 11 - Toxicological Information


RTECS#:      
CAS# 64-17-5: KQ6300000      
CAS# 67-66-3: FS9100000      
CAS# 64-19-7: AF1225000      
CAS# 141-78-6: AH5425000      
CAS# 67-56-1: PC1400000      
CAS# unlisted.
LD50/LC50:
CAS# 64-17-5:
     Draize test, rabbit, eye: 500 mg Severe;
     Draize test, rabbit, eye: 500 mg/24H Mild;
     Draize test, rabbit, skin: 20 mg/24H Moderate;
     Inhalation, mouse: LC50 = 39 gm/m3/4H;
     Inhalation, rat: LC50 = 20000 ppm/10H;
     Oral, mouse: LD50 = 3450 mg/kg;
     Oral, rabbit: LD50 = 6300 mg/kg;
     Oral, rat: LD50 = 7060 mg/kg;
     Oral, rat: LD50 = 9000 mg/kg;
.

CAS# 67-66-3:
     Draize test, rabbit, eye: 148 mg;
     Draize test, rabbit, eye: 20 mg/24H Moderate;
     Draize test, rabbit, skin: 500 mg/24H Mild;
     Inhalation, mouse: LC50 = 17200 mg/m3/2H;
     Inhalation, mouse: LC50 = 6000 mg/m3/6H;
     Inhalation, rat: LC50 = 47702 mg/m3/4H;
     Inhalation, rat: LC50 = 6000 mg/m3/6H;
     Oral, mouse: LD50 = 36 mg/kg;
     Oral, rat: LD50 = 695 mg/kg;
     Oral, rat: LD50 = 1250 mg/kg;
     Skin, rabbit: LD50 = >20 gm/kg;
.

CAS# 64-19-7:
     Draize test, rabbit, skin: 50 mg/24H Mild;
     Inhalation, mouse: LC50 = 5620 ppm/1H;
     Oral, rat: LD50 = 3310 mg/kg;
     Skin, rabbit: LD50 = 1060 uL/kg;
.

CAS# 141-78-6:
     Inhalation, mouse: LC50 = 45 gm/m3/2H;
     Inhalation, rat: LC50 = 200 gm/m3;
     Oral, mouse: LD50 = 4100 mg/kg;
     Oral, rabbit: LD50 = 4935 mg/kg;
     Oral, rat: LD50 = 5620 mg/kg;
     Skin, rabbit: LD50 = >20 mL/kg;
.

CAS# 67-56-1:
     Draize test, rabbit, eye: 40 mg Moderate;
     Draize test, rabbit, eye: 100 mg/24H Moderate;
     Draize test, rabbit, skin: 20 mg/24H Moderate;
     Inhalation, rabbit: LC50 = 81000 mg/m3/14H;
     Inhalation, rat: LC50 = 64000 ppm/4H;
     Oral, mouse: LD50 = 7300 mg/kg;
     Oral, rabbit: LD50 = 14200 mg/kg;
     Oral, rat: LD50 = 5600 mg/kg;
     Skin, rabbit: LD50 = 15800 mg/kg;
.

CAS# :
.

Carcinogenicity:
CAS# 64-17-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 67-66-3:

CAS# 64-19-7: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 141-78-6: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 67-56-1: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# : Not listed by ACGIH, IARC, NTP, or CA Prop 65.

Epidemiology: Prenatal exposure to ethanol is associated with a distinct pattern of congenital malformations that have collectively been termed the "fetal alcohol syndrome".Methanol has been shown to produce fetotoxicity in the embryo or fetus of laboratory animals. Specific developmental abnormalities include cardiovascular, musculoskeletal, and urogenital systems.Ethanol has been shown to produce fetotoxicity in the embryo or fetus of laboratory animals.
Teratogenicity: CAS# 64-17-5: Oral, Human - woman: TDLo = 41 gm/kg (female 41 week(s) after conception) Effects on Newborn - Apgar score (human only) and Effects on Newborn - other neonatal measures or effects and Effects on Newborn - drug dependence.
Reproductive Effects: CAS# 64-17-5: Intrauterine, Human - woman: TDLo = 200 mg/kg (female 5 day(s) pre-mating) Fertility - female fertility index (e.g. # females pregnant per # sperm positive females; # females pregnant per # females mated).
Mutagenicity: CAS# 64-17-5: DNA Inhibition: Human, Lymphocyte = 220 mmol/L.; Cytogenetic Analysis: Human, Lymphocyte = 1160 gm/L.; Cytogenetic Analysis: Human, Fibroblast = 12000 ppm.; Cytogenetic Analysis: Human, Leukocyte = 1 pph/72H (Continuous).; Sister Chromatid Exchange: Human, Lymphocyte = 500 ppm/72H (Continuous).
Neurotoxicity: No information found
Other Studies:

Section 12 - Ecological Information


Ecotoxicity: Fish: Rainbow trout: LC50 = 12900-15300 mg/L; 96 Hr; Flow-through @ 24-24.3°CFish: Rainbow trout: LC50 = 11200 mg/L; 24 Hr; Fingerling (Unspecified)Bacteria: Phytobacterium phosphoreum: EC50 = 34900 mg/L; 5-30 min; Microtox test CAS# 64-17-5: When spilled on land it is apt to volatilize, biodegrade, and leach into the ground water, but no data on the rates of these processes could be found. Its fate in ground water is unknown. When released into water it will volatilize and probably biodegrade. It would not be expected to adsorb to sediment or bioconcentrate in fish.
Environmental: CAS# 64-17-5: When released to the atmosphere it will photodegrade in hours (polluted urban atmosphere) to an estimated range of 4 to 6 days in less polluted areas. Rainout should be significant.
Physical: No information available.
Other: No information available.

Section 13 - Disposal Considerations

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series:
CAS# 67-66-3: waste number U044.
CAS# 141-78-6: waste number U112 (Ignitable waste).
CAS# 67-56-1: waste number U154 (Ignitable waste).

Section 14 - Transport Information

US DOT Canada TDG
Shipping Name: FLAMMABLE LIQUIDS, CORROSIVE, N.O.S. ETHANOL SOLUTION
Hazard Class: 3 3
UN Number: UN2924 UN1170
Packing Group: II II
Additional Info:FLASHPOINT 16C

Section 15 - Regulatory Information

US FEDERAL

TSCA
     CAS# 64-17-5 is listed on the TSCA inventory.
     CAS# 67-66-3 is listed on the TSCA inventory.
     CAS# 64-19-7 is listed on the TSCA inventory.
     CAS# 141-78-6 is listed on the TSCA inventory.
     CAS# 67-56-1 is listed on the TSCA inventory.
     CAS# is not listed on the TSCA inventory. It is for research and development use only.
Health & Safety Reporting List
     CAS# 67-66-3: Effective 6/1/87, Sunset 6/1/97
Chemical Test Rules
     CAS# 141-78-6: 40 CFR 799.5000
Section 12b
     None of the chemicals are listed under TSCA Section 12b.
TSCA Significant New Use Rule
     None of the chemicals in this material have a SNUR under TSCA.
CERCLA Hazardous Substances and corresponding RQs
     CAS# 67-66-3: 10 lb final RQ; 4.54 kg final RQ      CAS# 64-19-7: 5000 lb final RQ; 2270 kg final RQ      CAS# 141-78-6: 5000 lb final RQ; 2270 kg final RQ      CAS# 67-56-1: 5000 lb final RQ; 2270 kg final RQ
SARA Section 302 Extremely Hazardous Substances
     CAS# 67-66-3: 10000 lb TPQ
SARA Codes
     CAS # 64-17-5: immediate, delayed, fire.
     CAS # 67-66-3: immediate, delayed.
     CAS # 64-19-7: immediate, delayed, fire.
     CAS # 141-78-6: fire.
     CAS # 67-56-1: immediate, fire.
Section 313
     This material contains Chloroform (CAS# 67-66-3, 30.0%),which is subject to the reporting requirements of Section 313 of SARA Title III and 40 CFR Part 373.
     This material contains Methyl alcohol (CAS# 67-56-1, 2.1%),which is subject to the reporting requirements of Section 313 of SARA Title III and 40 CFR Part 373.
Clean Air Act:
     CAS# 67-66-3 is listed as a hazardous air pollutant (HAP).


     CAS# 67-56-1 is listed as a hazardous air pollutant (HAP).

     This material does not contain any Class 1 Ozone depletors.
     This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
CAS# 67-66-3 is listed as a Hazardous Substance under the CWA. CAS# 64-19-7 is listed as a Hazardous Substance under the CWA.      CAS# 67-66-3 is listed as a Priority Pollutant under the Clean Water      Act. CAS# 67-66-3 is listed as a Toxic Pollutant under the Clean Water Act.
OSHA:
     None of the chemicals in this product are considered highly hazardous by OSHA.
STATE
     CAS# 64-17-5 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.
     CAS# 67-66-3 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.
     CAS# 64-19-7 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.
     CAS# 141-78-6 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.
     CAS# 67-56-1 can be found on the following state right to know lists: California, New Jersey, Pennsylvania, Minnesota, Massachusetts.
     CAS# is not present on state lists from CA, PA, MN, MA, FL, or NJ.

California Prop 65
WARNING: This product contains Chloroform, a chemical known to the state of California to cause cancer. WARNING: This product contains Ethyl alcohol, a chemical known to the state of California to cause developmental reproductive toxicity.
California No Significant Risk Level: CAS# 67-66-3: 20 æg/day NSRL (oral); 40 æg/day NSRL (inhalation)

European/International Regulations

European Labeling in Accordance with EC Directives
Hazard Symbols:
     F
Risk Phrases:

     R 11 Highly flammable.

Safety Phrases:

     S 16 Keep away from sources of ignition - No smoking.
     S 33 Take precautionary measures against static discharges.
     S 7 Keep container tightly closed.
     S 9 Keep container in a well-ventilated place.

WGK (Water Danger/Protection)

     CAS# 64-17-5: 0
     CAS# 67-66-3: 3
     CAS# 64-19-7: 1
     CAS# 141-78-6: 1
     CAS# 67-56-1: 1
     CAS# : No information available.
Canada - DSL/NDSL
     CAS# 64-17-5 is listed on Canada's DSL List.
     CAS# 67-66-3 is listed on Canada's DSL List.
     CAS# 64-19-7 is listed on Canada's DSL List.
     CAS# 141-78-6 is listed on Canada's DSL List.
     CAS# 67-56-1 is listed on Canada's DSL List.
Canada - WHMIS
     This product has a WHMIS classification of B2, D2A.
This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations and the MSDS contains all of the information required by those regulations.
Canadian Ingredient Disclosure List
     CAS# 64-17-5 is listed on the Canadian Ingredient Disclosure List.
     CAS# 67-66-3 is listed on the Canadian Ingredient Disclosure List.
     CAS# 64-19-7 is listed on the Canadian Ingredient Disclosure List.
     CAS# 141-78-6 is listed on the Canadian Ingredient Disclosure List.
     CAS# 67-56-1 is listed on the Canadian Ingredient Disclosure List.

Section 16 - Additional Information

MSDS Creation Date: 4/26/1999
Revision #6 Date: 10/30/2007

The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Fisher be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Fisher has been advised of the possibility of such damages.