Material Safety Data Sheet
Phenol/Chloroform/Isoamyl Alcohol, pH 6.7/8.0
ACC# 91625
Section 1 - Chemical Product and Company Identification |
MSDS Name: Phenol/Chloroform/Isoamyl Alcohol, pH 6.7/8.0
Catalog Numbers:
BP1752I-100, BP1752I-400
Synonyms:
Phenol Chloroform Mixture II (25:24:1)
Company Identification:
Fisher Scientific
1 Reagent Lane
Fair Lawn, NJ 07410
For information, call: 201-796-7100
Emergency Number: 201-796-7100
For CHEMTREC assistance, call: 800-424-9300
For International CHEMTREC assistance, call: 703-527-3887
Section 2 - Composition, Information on Ingredients
|
CAS# |
Chemical Name |
Percent |
EINECS/ELINCS |
108-95-2 |
Phenol |
45-55% | 203-632-7 |
67-66-3 |
Chloroform |
45-50% | 200-663-8 |
123-51-3 |
Isoamyl alcohol |
1.5-2.5% | 204-633-5 |
Section 3 - Hazards Identification
|
EMERGENCY OVERVIEW
Appearance: clear, colorless liquid.
Danger! Causes irritation and possible burns by
all routes of exposure. Corrosive. Harmful if swallowed or absorbed
through the skin. May be harmful if inhaled. Potential cancer
hazard. May cause central nervous system depression. May cause liver
and kidney damage. May cause methemoglobinemia. Keep refrigerated.
(Store below 4°C/39°F.) May cause fetal effects based upon animal
studies.
Target Organs: Blood, kidneys, central nervous
system, liver, respiratory system, eyes, skin.
Potential Health Effects
Eye:
Causes eye irritation and possible burns. May cause chemical
conjunctivitis and corneal damage.
Skin:
Harmful if absorbed through the skin.
Direct skin contact may result in white, wrinkled discoloration,
followed by severe burns. Phenol solutions may be absorbed through
the skin rapidly to cause systemic poisoning and possible death.
Ingestion:
Harmful if swallowed. May cause central nervous system depression,
characterized by excitement, followed by headache, dizziness,
drowsiness, and nausea. Advanced stages may cause collapse,
unconsciousness, coma and possible death due to respiratory failure.
Overexposure may cause methemoglobinemia. May cause systemic effects.
Causes digestive tract irritation with possible burns.
Inhalation:
May be fatal if exposed to high concentrations. May cause severe
respiratory tract irritation and possible burns. Aspiration may lead
to pulmonary edema. May also cause pallor, loss of appetite, nausea,
vomiting, diarrhea, weakness, darkened urine, headache, sweating,
convulsions, cyanosis (bluish skin due to deficient oxygenation of
the blood), unconsciousness, fatigue, pulmonary edema & coma. May
cause systemic effects. Inhalation at high concentrations may cause
CNS depression and asphixiation.
Chronic:
May cause liver and kidney damage. May cause fetal effects. Effects
may be delayed. Repeated skin contact may cause dermatitis with dark
pigmentation of the skin. Chronic exposure has been associated with
an increased incidence of kidney, liver, rectal, bladder, colon,
brain, and lymph node cancer.
Section 4 - First Aid Measures
|
Eyes:
Get medical aid immediately. Do NOT allow victim to rub eyes or keep
eyes closed. Extensive irrigation with water is required (at least 30
minutes).
Skin:
Get medical aid immediately. Immediately flush skin with plenty of
water for at least 15 minutes while removing contaminated clothing
and shoes. SPEEDY ACTION IS CRITICAL! Destroy contaminated shoes.
Ingestion:
Do not induce vomiting. If victim is conscious and alert, give 2-4
cupfuls of milk or water. Never give anything by mouth to an
unconscious person. Get medical aid immediately.
Inhalation:
Get medical aid immediately. Remove from exposure and move to fresh
air immediately. If breathing is difficult, give oxygen. Do NOT use
mouth-to-mouth resuscitation. If breathing has ceased apply
artificial respiration using oxygen and a suitable mechanical device
such as a bag and a mask.
Notes to Physician:
Persons with liver or kidney disease should not be exposed to phenol
for any length of time.
Section 5 - Fire Fighting Measures
|
General Information:
As in any fire, wear a self-contained breathing apparatus in
pressure-demand, MSHA/NIOSH (approved or equivalent), and full
protective gear. During a fire, irritating and highly toxic gases
may be generated by thermal decomposition or combustion. Use water
spray to keep fire-exposed containers cool. Containers may explode
when heated.
Extinguishing Media:
For small fires, use dry chemical, carbon dioxide, water spray or
alcohol-resistant foam. Use water spray to cool fire-exposed
containers. Do NOT get water inside containers. For large fires, use
dry chemical, carbon dioxide, alcohol-resistant foam, or water
spray. Cool containers with flooding quantities of water until well
after fire is out.
Flash Point: Not applicable.
Autoignition Temperature: Not applicable.
Explosion Limits, Lower:Not available.
Upper: Not available.
NFPA Rating:
(estimated) Health: 4; Flammability: 1; Instability: 0
Section 6 - Accidental Release Measures
|
General Information: Use proper personal protective equipment as indicated
in Section 8.
Spills/Leaks:
Absorb spill with inert material (e.g. vermiculite, sand or earth),
then place in suitable container. Clean up spills immediately,
observing precautions in the Protective Equipment section. Provide
ventilation.
Section 7 - Handling and Storage
|
Handling:
Wash thoroughly after handling. Remove contaminated clothing and
wash before reuse. Use only in a well-ventilated area. Do not get in
eyes, on skin, or on clothing. Keep container tightly closed. Do not
ingest or inhale. Discard contaminated shoes.
Storage:
Keep container closed when not in use. Store in a tightly closed
container. Keep from contact with oxidizing materials. Store in a
cool, dry, well-ventilated area away from incompatible substances.
Keep refrigerated. (Store below 4°C/39°F.)
Section 8 - Exposure Controls, Personal Protection
|
Engineering Controls:
Use explosion-proof ventilation equipment. Facilities storing or
utilizing this material should be equipped with an eyewash facility
and a safety shower. Use adequate general or local exhaust
ventilation to keep airborne concentrations below the permissible
exposure limits.
Exposure Limits
Chemical Name |
ACGIH |
NIOSH |
OSHA - Final PELs |
Phenol
|
5 ppm TWA; Skin - potential significant contribution to overall exposure by the cutaneous r oute
|
5 ppm TWA; 19 mg/m3 TWA 250 ppm IDLH
|
5 ppm TWA; 19 mg/m3 TWA
|
Chloroform
|
10 ppm TWA
|
500 ppm IDLH
|
50 ppm Ceiling; 240 mg/m3 Ceiling
|
Isoamyl alcohol
|
100 ppm TWA; 125 ppm STEL
|
100 ppm TWA; 360 mg/m3 TWA 500 ppm IDLH
|
100 ppm TWA; 360 mg/m3 TWA
|
OSHA Vacated PELs:
Phenol:
5 ppm TWA; 19 mg/m3 TWA
Chloroform:
2 ppm TWA; 9.78 mg/m3 TWA
Isoamyl alcohol:
100 ppm TWA; 360 mg/m3 TWA
Personal Protective Equipment
Eyes:
Wear appropriate protective eyeglasses or chemical
safety goggles as described by OSHA's eye and face
protection regulations in 29 CFR 1910.133 or European
Standard EN166.
Skin:
Wear appropriate protective gloves to prevent skin
exposure.
Clothing:
Wear appropriate protective clothing to prevent skin
exposure.
Respirators:
A respiratory protection program that meets OSHA's 29
CFR 1910.134 and ANSI Z88.2 requirements or European
Standard EN 149 must be followed whenever workplace
conditions warrant respirator use.
Section 9 - Physical and Chemical Properties
|
Physical State: Liquid
Appearance: clear, colorless
Odor: none reported
pH: 6.7-8.0
Vapor Pressure: Not available.
Vapor Density: 5.59
Evaporation Rate:Not available.
Viscosity: Not available.
Boiling Point: 96 - 97 deg C @ 8.30 mmHg
Freezing/Melting Point:11 - 12 deg C
Decomposition Temperature:Not available.
Solubility: Not available.
Specific Gravity/Density:1.1800g/cm3
Molecular Formula:C6H10O5
Molecular Weight:162.14
Section 10 - Stability and Reactivity
|
Chemical Stability:
Stable under normal temperatures and pressures.
Conditions to Avoid:
Excess heat.
Incompatibilities with Other Materials:
Metals, strong oxidizing agents, reducing agents, acids, acid
chlorides, aluminum, fluorine, magnesium, isocyanates, potassium,
acetaldehyde, lithium, sodium, nitrides (e.g. potassium nitride,
sodium nitride), acid anhydrides, calcium hypochlorite, dinitrogen
tetraoxide, sodium + methanol, peroxomonosulfuric acid, potassium
tert-butoxide, nitrobenzene, sodium nitrite, aluminum chloride,
peroxydisulfuric acid, acetone + alkali, disilane, sodium methylate,
triisopropylphosphine, sodium methoxide + methanol, hydrogen
trisulfide, 1,3-butadiene, boron trifluoride diethyl ether.
Hazardous Decomposition Products:
Hydrogen chloride, phosgene, carbon monoxide, irritating and toxic
fumes and gases, carbon dioxide, chlorine.
Hazardous Polymerization: Has not been reported.
Section 11 - Toxicological Information
|
RTECS#:
CAS# 108-95-2:
SJ3325000
CAS# 67-66-3:
FS9100000
CAS# 123-51-3:
EL5425000
LD50/LC50:
CAS# 108-95-2:
Draize test,
rabbit, eye: 5 mg Severe;
Draize
test, rabbit, skin: 500 mg/24H Severe;
Draize test, rabbit, skin: 100 mg
Mild;
Inhalation, mouse: LC50 =
177 mg/m3;
Inhalation, mouse: LC50
= 177 mg/m3/4H;
Inhalation, rat:
LC50 = 316 mg/m3;
Inhalation, rat:
LC50 = 316 mg/m3/4H;
Oral, mouse:
LD50 = 270 mg/kg;
Oral, rat: LD50 =
317 mg/kg;
Oral, rat: LD50 = 512
mg/kg;
Skin, rabbit: LD50 = 630
mg/kg;
Skin, rat: LD50 = 669
mg/kg;
Skin, rat: LD50 = 1500
mg/kg;
.
CAS# 67-66-3:
Draize test,
rabbit, eye: 148 mg;
Draize test,
rabbit, eye: 20 mg/24H Moderate;
Draize test, rabbit, skin: 500 mg/24H
Mild;
Inhalation, mouse: LC50 =
17200 mg/m3/2H;
Inhalation, mouse:
LC50 = 6000 mg/m3/6H;
Inhalation,
rat: LC50 = 47702 mg/m3/4H;
Inhalation, rat: LC50 = 6000
mg/m3/6H;
Oral, mouse: LD50 = 36
mg/kg;
Oral, rat: LD50 = 695
mg/kg;
Oral, rat: LD50 = 1250
mg/kg;
Skin, rabbit: LD50 = >20
gm/kg;
.
CAS# 123-51-3:
Draize test,
rabbit, eye: 20 mg/24H Moderate;
Draize test, rabbit, skin: 20 mg/24H
Moderate;
Oral, rabbit: LD50 =
3438 mg/kg;
Oral, rat: LD50 = 1300
mg/kg;
Oral, rat: LD50 = 4300
mg/kg;
Skin, rabbit: LD50 = 3970
uL/kg;
.
Carcinogenicity:
CAS# 108-95-2:
Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 67-66-3:
- ACGIH:
A3 - Confirmed animal carcinogen with unknown relevance to humans
- California:
carcinogen, initial date 10/1/87
- NTP:
Suspect carcinogen
- IARC:
Group 2B carcinogen
CAS# 123-51-3:
Not listed by ACGIH, IARC, NTP, or CA Prop 65.
Epidemiology:
No data available.
Teratogenicity:
CAS# 108-95-2: Oral, rat: TDLo = 1200 mg/kg (female 6-15
day(s) after conception) Effects on Embryo or Fetus -
fetotoxicity (except death, e.g., stunted fetus).; Oral,
mouse: TDLo = 4 gm/kg (female 6-15 day(s) after conception)
Specific Developmental Abnormalities - musculoskeletal
system.
Reproductive Effects:
CAS# 108-95-2: Oral, rat: TDLo = 300 mg/kg (female 6-15
day(s) after conception) Fertility - post-implantation
mortality (e.g. dead and/or resorbed implants per total
number of implants).
Mutagenicity:
CAS# 108-95-2: Mutation Test Systems - not otherwise
specified: Human, HeLa cell = 17 mg/L.; DNA Inhibition:
Human, HeLa cell = 1 mmol/L.; Mutation Test Systems - not
otherwise specified: Human, Lymphocyte = 5 umol/L.; Sister
Chromatid Exchange: Human, Lymphocyte = 5 umol/L.
Neurotoxicity:
No information found
Other Studies:
Section 12 - Ecological Information
|
Ecotoxicity:
Water flea Daphnia: EC50=12 mg/l; 48-hour; CAS# 108-95-2:
UnspecifiedWater flea Daphnia: EC50=4.0 mg/l; 96-hour; CAS#
108-95-2: UnspecifiedFish: Fathead Minnow: LC50 > 50 mg/l; 1 Hr;
CAS# 108-95-2 Static @ 18-22°CFish: Fathead Minnow: TLm = 41
mg/L; 48-hour; CAS# 108-95-2: Flow-through @ 15°CFish:
Bluegill/Sunfish: TLm = 19 / 5.7 mg/L; 96 Hr; CAS# 108-95-2:
Flow-through
If released to the environment, phenol's primary removal mechanism is
biodegradation which is generally rapid (days). If phenol is released
to soil, it will readily leach and biodegrade. The biodegradation in
soil is generally rapid with half-lives of under 5 days even in
subsurface soils.
Environmental:
Phenol does not bioconcentrate in aquatic organisms. In the
atmosphere, phenol occurs as a vapor and reacts with
photochemically-produced hydroxyl radicals resulting in a half-life
of approximately 15 hours. During the nighttime, it reacts with
nitrate radicals with a resulting half-life of 12 minutes. Phenol
has also been shown to be readily removed from the atmosphere by
rain.
Physical:
No information available.
Other:
No information available.
Section 13 - Disposal Considerations
|
Chemical waste generators must determine whether a discarded chemical is classified
as a hazardous waste.
US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3.
Additionally, waste generators must consult state and local hazardous waste regulations to
ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series:
CAS# 108-95-2: waste
number U188.
CAS# 67-66-3: waste number U044.
Section 14 - Transport Information
|
|
US DOT |
Canada TDG |
Shipping Name: |
TOXIC LIQUIDS, ORGANIC, N.O.S. |
TOXIC LIQUIDS, ORGANIC, N.O.S. (Phenol, Chloroform) |
Hazard Class: |
6.1 |
6.1 |
UN Number: |
UN2810 |
UN2810 |
Packing Group: |
II |
II |
Section 15 - Regulatory Information
|
US FEDERAL
TSCA
CAS# 108-95-2 is listed on the TSCA inventory.
CAS# 67-66-3 is listed on the TSCA inventory.
CAS# 123-51-3 is listed on the TSCA inventory.
Health & Safety Reporting List
CAS# 108-95-2: Effective 6/1/87, Sunset 6/1/97
CAS# 67-66-3: Effective 6/1/87, Sunset 6/1/97
Chemical Test Rules
None of the chemicals in this product are under a Chemical Test Rule.
Section 12b
None of the chemicals are listed under TSCA Section 12b.
TSCA Significant New Use Rule
None of the chemicals in this material have a SNUR under TSCA.
CERCLA Hazardous Substances and corresponding RQs
CAS# 108-95-2: 1000 lb final RQ; 454 kg final RQ
CAS# 67-66-3: 10 lb final RQ; 4.54 kg final RQ
SARA Section 302 Extremely Hazardous Substances
CAS# 108-95-2: 500 lb lower threshold TPQ; 10000 lb upper threshold TP
Q
CAS# 67-66-3: 10000 lb TPQ
SARA Codes
CAS # 108-95-2: immediate, delayed, fire.
CAS # 67-66-3: immediate, delayed.
CAS # 123-51-3: immediate, fire.
Section 313
This material contains Phenol (CAS#
108-95-2, 45-55%),which is subject to the reporting requirements of
Section 313 of SARA Title III and 40 CFR Part 373.
This material contains Chloroform
(CAS# 67-66-3, 45-50%),which is subject to the reporting requirements
of Section 313 of SARA Title III and 40 CFR Part 373.
Clean Air Act:
CAS# 108-95-2 is listed as a hazardous air pollutant (HAP).
CAS# 67-66-3 is listed as a hazardous air pollutant (HAP).
This material does not contain any Class 1 Ozone depletors.
This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
CAS# 108-95-2 is listed as a Hazardous Substance under the CWA.
CAS# 67-66-3 is listed as a Hazardous Substance under the CWA.
CAS# 108-95-2 is listed as a Priority Pollutant under the Clean Water
Act.
CAS# 67-66-3 is listed as a Priority Pollutant under the Clean Water
Act.
CAS# 108-95-2 is listed as a Toxic Pollutant under the Clean Water
Act.
CAS# 67-66-3 is listed as a Toxic Pollutant under the Clean Water
Act.
OSHA:
None of the chemicals in this product
are considered highly hazardous by OSHA.
STATE
CAS# 108-95-2 can be found on the
following state right to know lists: California, New Jersey,
Pennsylvania, Minnesota, Massachusetts.
CAS# 67-66-3 can be found on the
following state right to know lists: California, New Jersey,
Pennsylvania, Minnesota, Massachusetts.
CAS# 123-51-3 can be found on the
following state right to know lists: California, New Jersey,
Pennsylvania, Minnesota, Massachusetts.
California Prop 65
WARNING: This product contains Chloroform, a chemical known to the
state of California to cause cancer.
California No Significant Risk Level:
CAS# 67-66-3: 20 æg/day NSRL (oral); 40 æg/day NSRL (inhalation)
European/International Regulations
European Labeling in Accordance with EC Directives
Hazard Symbols:
T C
Risk Phrases:
R 23/24/25 Toxic by inhalation, in contact with skin and if
swallowed.
R 34 Causes burns.
R 40 Limited evidence of a carcinogenic effect.
R 48/20/21/22 Harmful : danger of serious damage to health by
prolonged exposure through inhalation, in contact with skin and if
swallowed.
R 68 Possible risk of irreversible effects.
Safety Phrases:
S 36/37 Wear suitable protective clothing and gloves.
S 45 In case of accident or if you feel unwell, seek medical advice
immediately (show the label where possible).
S 28A After contact with skin, wash immediately with plenty of water
.
WGK (Water Danger/Protection)
CAS# 108-95-2: 2
CAS# 67-66-3: 3
CAS# 123-51-3: 1
Canada - DSL/NDSL
CAS# 108-95-2 is listed on Canada's DSL List.
CAS# 67-66-3 is listed on Canada's DSL List.
CAS# 123-51-3 is listed on Canada's DSL List.
Canada - WHMIS
This product has a WHMIS classification of D1A, D2A, E.
This product has been classified in accordance with the hazard
criteria of the Controlled Products Regulations and the MSDS
contains all of the information required by those regulations.
Canadian Ingredient Disclosure List
CAS# 108-95-2 is listed on the Canadian Ingredient Disclosure List.
CAS# 67-66-3 is listed on the Canadian Ingredient Disclosure List.
CAS# 123-51-3 is listed on the Canadian Ingredient Disclosure List.
Section 16 - Additional Information
|
MSDS Creation Date: 8/03/2000
Revision #5 Date: 6/29/2007
The information above is believed to be accurate and represents the best
information currently available to us. However, we make no warranty of
merchantability or any other warranty, express or implied, with respect to
such information, and we assume no liability resulting from its use. Users
should make their own investigations to determine the suitability of the
information for their particular purposes. In no event shall Fisher be liable
for any claims, losses, or damages of any third party or for lost profits
or any special, indirect, incidental, consequential or exemplary
damages, howsoever arising, even if Fisher has been advised of
the possibility of such damages.