Material Safety Data Sheet
Malachite Green (Oxalate) Solution

ACC# 95197
Section 1 - Chemical Product and Company Identification

MSDS Name: Malachite Green (Oxalate) Solution
Catalog Numbers: S713161
Synonyms:
Acryl Brilliant Green; Aniline Green; China Green; Fast Green; Victoria Green
Company Identification:

              Fisher Scientific
              1 Reagent Lane
              Fair Lawn, NJ 07410
For information, call:
201-796-7100
Emergency Number:
201-796-7100
For CHEMTREC assistance, call:
800-424-9300
For International CHEMTREC assistance, call:
703-527-3887

Section 2 - Composition, Information on Ingredients

CAS# Chemical Name Percent EINECS/ELINCS
7732-18-5 Water 99 % 231-791-2
2437-29-8 Malachite green oxalate 1.0 % 219-441-7

Section 3 - Hazards Identification

EMERGENCY OVERVIEW

Appearance: almost colorless liquid.
Warning! May cause digestive tract irritation with nausea, vomiting, and diarrhea. May cause skin irritation. Eye contact may result in permanent eye damage. May cause fetal effects. The toxicological properties of this material have not been fully investigated.
Target Organs: None known.


Potential Health Effects
Eye: May cause severe eye irritation. This product contains a cationic dye. Similar dyes have caused permanent injury to the cornea and conjunctiva in documented exposure cases with human or rabbit eyes.
Skin: May cause skin irritation.
Ingestion: May cause gastrointestinal irritation with nausea, vomiting and diarrhea. The toxicological properties of this substance have not been fully investigated.
Inhalation: The toxicological properties of this substance have not been fully investigated.
Chronic: May cause fetal effects.

Section 4 - First Aid Measures


Eyes: Immediately flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. Get medical aid imme diately.
Skin: Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Get medical aid if irritation develops or persists. Wash clothing before reuse.
Ingestion: Do not induce vomiting. If victim is conscious and alert, give 2-4 cupfuls of milk or water. Get medical aid.
Inhalation: Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid if cough or other symptoms appear.
Notes to Physician: Treat symptomatically and supportively.

Section 5 - Fire Fighting Measures


General Information: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Use extinguishing media appropriate to the surrounding fire. Substance is noncombustible.
Extinguishing Media: For small fires, use water spray, dry chemical, carbon dioxide or chemical foam. Substance is noncombustible; use agent most appropriate to extinguish surrounding fire.
Flash Point: Not applicable.
Autoignition Temperature: Not applicable.
Explosion Limits, Lower:Not available.
Upper: Not available.
NFPA Rating: (estimated) Health: 2; Flammability: 0; Instability: 0

Section 6 - Accidental Release Measures


General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: Absorb spill with inert material (e.g. vermiculite, sand or earth), then place in suitable container. Avoid runoff into storm sewers and ditches which lead to waterways. Clean up spills immediately, observing precautions in the Protective Equipment section. Avoid generating dusty conditions. Provide ventilation.

Section 7 - Handling and Storage


Handling: Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Use with adequate ventilation. Avoid contact with skin and eyes. Keep container tightly closed. Avoid ingestion and inhalation.
Storage: Store in a cool, dry place.

Section 8 - Exposure Controls, Personal Protection


Engineering Controls: Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate ventilation to keep airborne concentrations low.
Exposure Limits
Chemical Name ACGIH NIOSH OSHA - Final PELs
Water none listed none listed none listed
Malachite green oxalate none listed none listed none listed

OSHA Vacated PELs: Water: No OSHA Vacated PELs are listed for this chemical. Malachite green oxalate: No OSHA Vacated PELs are listed for this chemical.
Personal Protective Equipment
Eyes: Wear appropriate protective eyeglasses or chemical safety goggles as described by OSHA's eye and face protection regulations in 29 CFR 1910.133 or European Standard EN166.
Skin: Wear appropriate gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.

Section 9 - Physical and Chemical Properties


Physical State: Liquid
Appearance: almost colorless
Odor: None reported
pH: Not available.
Vapor Pressure: Not available.
Vapor Density: >1.0
Evaporation Rate:Not available.
Viscosity: Not available.
Boiling Point: 0 deg C
Freezing/Melting Point:0 deg C
Decomposition Temperature:Not available.
Solubility: Not available.
Specific Gravity/Density:Not available.
Molecular Formula:C23H25N2Cl
Molecular Weight:364.6414

Section 10 - Stability and Reactivity


Chemical Stability: Stable under normal temperatures and pressures.
Conditions to Avoid: High temperatures.
Incompatibilities with Other Materials: No information available.
Hazardous Decomposition Products: Carbon monoxide, oxides of nitrogen, carbon dioxide.
Hazardous Polymerization: Has not been reported

Section 11 - Toxicological Information


RTECS#:      
CAS# 7732-18-5: ZC0110000      
CAS# 2437-29-8: BQ1190000
LD50/LC50:
CAS# 7732-18-5:
     Oral, rat: LD50 = >90 mL/kg;
.

CAS# 2437-29-8:
     Draize test, rabbit, eye: 76 mg Severe;
     Oral, rat: LD50 = 275 mg/kg;
.

Carcinogenicity:
CAS# 7732-18-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.
CAS# 2437-29-8: Not listed by ACGIH, IARC, NTP, or CA Prop 65.

Epidemiology: Malachite green is similar in structure to other carcinogenic dyes. It seems to enhance the formation of hepatic tumors in rats. It appears to act as a tumor promoter.
Teratogenicity: Significant teratological effects were noted in rabbits administered 0, 5,10, or 20 mg of malachite green/kg body weight by gavage on days 6 through 18 of gestation. There were significant increases in preimplantation losses. Skeletal deviations were the most common abnormality. Also, enlargement of the liver, heart, and abdominal cavity was observed.
Reproductive Effects: No information available.
Mutagenicity: Malachite green is mutagenic is Salmonella typhimurium in the presence of an exogenous metabolizing system. It shows a marked cytotoxicity and the ability to induce cell transformation and lipid peroxidation in mammalian cells.
Neurotoxicity: No information available.
Other Studies:

Section 12 - Ecological Information


Ecotoxicity: No data available. No information available.
Environmental: No information reported.
Physical: No information available.
Other: No information available.

Section 13 - Disposal Considerations

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series: None listed.

Section 14 - Transport Information

US DOT Canada TDG
Shipping Name: Not regulated as a hazardous material No information available.
Hazard Class:
UN Number:
Packing Group:

Section 15 - Regulatory Information

US FEDERAL

TSCA
     CAS# 7732-18-5 is listed on the TSCA inventory.
     CAS# 2437-29-8 is not listed on the TSCA inventory. It is for research and development use only.
Health & Safety Reporting List
     None of the chemicals are on the Health & Safety Reporting List.
Chemical Test Rules
     None of the chemicals in this product are under a Chemical Test Rule.
Section 12b
     None of the chemicals are listed under TSCA Section 12b.
TSCA Significant New Use Rule
     None of the chemicals in this material have a SNUR under TSCA.
CERCLA Hazardous Substances and corresponding RQs
     None of the chemicals in this material have an RQ.
SARA Section 302 Extremely Hazardous Substances
     None of the chemicals in this product have a TPQ.
SARA Codes
     CAS # 2437-29-8: immediate.
Section 313      No chemicals are reportable under Section 313.
Clean Air Act:
     This material does not contain any hazardous air pollutants.
     This material does not contain any Class 1 Ozone depletors.
     This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
     None of the chemicals in this product are listed as Hazardous Substances under the CWA.
     None of the chemicals in this product are listed as Priority Pollutants under the CWA.
     None of the chemicals in this product are listed as Toxic Pollutants under the CWA.
OSHA:
     None of the chemicals in this product are considered highly hazardous by OSHA.
STATE
     CAS# 7732-18-5 is not present on state lists from CA, PA, MN, MA, FL, or NJ.
     CAS# 2437-29-8 is not present on state lists from CA, PA, MN, MA, FL, or NJ.

California Prop 65

California No Significant Risk Level: None of the chemicals in this product are listed.

European/International Regulations

European Labeling in Accordance with EC Directives
Hazard Symbols:
      Not available.
Risk Phrases:


Safety Phrases:


WGK (Water Danger/Protection)

     CAS# 7732-18-5: No information available.
     CAS# 2437-29-8: 3
Canada - DSL/NDSL
     CAS# 7732-18-5 is listed on Canada's DSL List.
     CAS# 2437-29-8 is listed on Canada's DSL List.
Canada - WHMIS
     WHMIS: Not available.
This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations and the MSDS contains all of the information required by those regulations.
Canadian Ingredient Disclosure List

Section 16 - Additional Information

MSDS Creation Date: 7/15/1999
Revision #3 Date: 10/03/2005

The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Fisher be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Fisher has been advised of the possibility of such damages.