Material Safety Data Sheet
Tris EDTA acetate buffer, 10x, DNase, RNase and Protease free

ACC# 99574
Section 1 - Chemical Product and Company Identification
MSDS Name: Tris EDTA acetate buffer, 10x, DNase, RNase and Protease free
Catalog Numbers: AC327350000, AC327355000
2-Amino-2-(hydroxymethyl)-1,3-propanediol; TRIS; Tromethane; Tromethamine; Tris buffer
Company Identification:

              Acros Organics N.V.
              One Reagent Lane
              Fair Lawn, NJ 07410
For information in North America, call: 800-ACROS-01
For emergencies in the US, call CHEMTREC: 800-424-9300

Section 2 - Composition, Information on Ingredients

CAS# Chemical Name Percent EINECS/ELINCS
135852-26-5 Tris EDTA acetate buffer, 10x, DNase, RNase and Protease free 100 unlisted

Section 3 - Hazards Identification


Appearance: colorless clear liquid.
Caution! Good laboratory procedures are recommended when handling this compound. This is expected to be a low hazard for usual industrial handling.
Target Organs: None known.

Potential Health Effects
Eye: May cause eye irritation.
Skin: May cause skin irritation.
Ingestion: May cause irritation of the digestive tract. Low hazard for usual industrial handling.
Inhalation: May cause respiratory tract irritation. Low hazard for usual industrial handling.
Chronic: Not expected to be a chronic hazard.

Section 4 - First Aid Measures

Eyes: Flush eyes with plenty of water for at least 15 minutes, occasionally lifting the upper and lower eyelids. If irritation develops, get medi cal aid.
Skin: Flush skin with plenty of water for at least 15 minutes while removing contaminated clothing and shoes. Get medical aid if irritation develops or persists.
Ingestion: If victim is conscious and alert, give 2-4 cupfuls of milk or water. Wash mouth out with water. Get medical aid if irritation or symptoms occur.
Inhalation: Remove from exposure and move to fresh air immediately. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical aid if cough or other symptoms appear.
Notes to Physician: Treat symptomatically and supportively.

Section 5 - Fire Fighting Measures

General Information: As in any fire, wear a self-contained breathing apparatus in pressure-demand, MSHA/NIOSH (approved or equivalent), and full protective gear. During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion.
Extinguishing Media: Use water spray, dry chemical, carbon dioxide, or chemical foam.
Flash Point: Not available.
Autoignition Temperature: Not available.
Explosion Limits, Lower:Not available.
Upper: Not available.
NFPA Rating: (estimated) Health: 1; Flammability: 0; Instability: 0

Section 6 - Accidental Release Measures

General Information: Use proper personal protective equipment as indicated in Section 8.
Spills/Leaks: Absorb spill with inert material (e.g. vermiculite, sand or earth), then place in suitable container. Clean up spills immediately, observing precautions in the Protective Equipment section. Provide ventilation.

Section 7 - Handling and Storage

Handling: Provide ventilation. Avoid breathing dust, mist, or vapor. Avoid contact with skin and eyes. Use with adequate ventilation.
Storage: Store in a cool, dry place. Store in a tightly closed container.

Section 8 - Exposure Controls, Personal Protection

Engineering Controls: Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower. Use adequate ventilation to keep airborne concentrations low.
Exposure Limits
Chemical Name ACGIH NIOSH OSHA - Final PELs
Tris EDTA acetate buffer, 10x, DNase, RNase and Protease free none listed none listed none listed

OSHA Vacated PELs: Tris EDTA acetate buffer, 10x, DNase, RNase and Protease free: No OSHA Vacated PELs are listed for this chemical.
Personal Protective Equipment
Eyes: Wear chemical splash goggles.
Skin: Wear appropriate protective gloves to prevent skin exposure.
Clothing: Wear appropriate protective clothing to prevent skin exposure.
Respirators: Follow the OSHA respirator regulations found in 29 CFR 1910.134 or European Standard EN 149. Use a NIOSH/MSHA or European Standard EN 149 approved respirator if exposure limits are exceeded or if irritation or other symptoms are experienced.

Section 9 - Physical and Chemical Properties

Physical State: Clear liquid
Appearance: colorless
Odor: odorless
pH: 8.2-8.4
Vapor Pressure: Not available.
Vapor Density: Not available.
Evaporation Rate:Not available.
Viscosity: Not available.
Boiling Point: 219 - 220 deg C @ 1
Freezing/Melting Point:170 deg C
Decomposition Temperature:Not available.
Solubility: Not available.
Specific Gravity/Density:Not available.
Molecular Formula:Solution
Molecular Weight:Not available.

Section 10 - Stability and Reactivity

Chemical Stability: Stable under normal temperatures and pressures.
Conditions to Avoid: Incompatible materials.
Incompatibilities with Other Materials: Strong oxidizing agents, strong acids, bases.
Hazardous Decomposition Products: Carbon monoxide, oxides of nitrogen, carbon dioxide.
Hazardous Polymerization: Will not occur.

Section 11 - Toxicological Information

CAS# 135852-26-5 unlisted.
Not available.

CAS# 135852-26-5: Not listed by ACGIH, IARC, NTP, or CA Prop 65.

Epidemiology: No information found
Teratogenicity: No information found
Reproductive Effects: No information found
Mutagenicity: No information found
Neurotoxicity: No information found
Other Studies:

Section 12 - Ecological Information

No information available.

Section 13 - Disposal Considerations

Chemical waste generators must determine whether a discarded chemical is classified as a hazardous waste. US EPA guidelines for the classification determination are listed in 40 CFR Parts 261.3. Additionally, waste generators must consult state and local hazardous waste regulations to ensure complete and accurate classification.
RCRA P-Series: None listed.
RCRA U-Series: None listed.

Section 14 - Transport Information

Shipping Name: Not regulated as a hazardous material Not Regulated
Hazard Class:
UN Number:
Packing Group:

Section 15 - Regulatory Information


     CAS# 135852-26-5 is not listed on the TSCA inventory. It is for research and development use only.
Health & Safety Reporting List
     None of the chemicals are on the Health & Safety Reporting List.
Chemical Test Rules
     None of the chemicals in this product are under a Chemical Test Rule.
Section 12b
     None of the chemicals are listed under TSCA Section 12b.
TSCA Significant New Use Rule
     None of the chemicals in this material have a SNUR under TSCA.
CERCLA Hazardous Substances and corresponding RQs
     None of the chemicals in this material have an RQ.
SARA Section 302 Extremely Hazardous Substances
     None of the chemicals in this product have a TPQ.
Section 313      No chemicals are reportable under Section 313.
Clean Air Act:
     This material does not contain any hazardous air pollutants.
     This material does not contain any Class 1 Ozone depletors.
     This material does not contain any Class 2 Ozone depletors.
Clean Water Act:
     None of the chemicals in this product are listed as Hazardous Substances under the CWA.
     None of the chemicals in this product are listed as Priority Pollutants under the CWA.
     None of the chemicals in this product are listed as Toxic Pollutants under the CWA.
     None of the chemicals in this product are considered highly hazardous by OSHA.
     CAS# 135852-26-5 is not present on state lists from CA, PA, MN, MA, FL, or NJ.

California Prop 65

California No Significant Risk Level: None of the chemicals in this product are listed.

European/International Regulations

European Labeling in Accordance with EC Directives
Hazard Symbols:
      Not available.
Risk Phrases:

Safety Phrases:

     S 24/25 Avoid contact with skin and eyes.

WGK (Water Danger/Protection)

     CAS# 135852-26-5: No information available.
Canada - DSL/NDSL
None of the chemicals in this product are listed on the DSL or NDSL list.
Canada - WHMIS
     not available.
This product has been classified in accordance with the hazard criteria of the Controlled Products Regulations and the MSDS contains all of the information required by those regulations.
Canadian Ingredient Disclosure List

Section 16 - Additional Information

MSDS Creation Date: 5/27/1998
Revision #5 Date: 11/20/2008

The information above is believed to be accurate and represents the best information currently available to us. However, we make no warranty of merchantability or any other warranty, express or implied, with respect to such information, and we assume no liability resulting from its use. Users should make their own investigations to determine the suitability of the information for their particular purposes. In no event shall Fisher be liable for any claims, losses, or damages of any third party or for lost profits or any special, indirect, incidental, consequential or exemplary damages, howsoever arising, even if Fisher has been advised of the possibility of such damages.